Isolation of Proteins which Interact with Phospholipase A2 (IIA) from Human Serum after Myocardial Infarction. Flavonoids from Bidens tripartita Extract as Phospholipase A2 Inhibitors

Abstract

The aim of the work was to isolate proteins which interact with phospholipase A₂ (PLA₂) from human serum after myocardial infarction. During this study, the effect of flavonoid inhibitors from the extract of Bidens Tripartita was examined. First, the paper describes phytochemical characterisation of compounds found in the plant Bidens tripartita.  Plant material was harvested at different vegetation stages, and extracts of each were studied for presence of flavonoids by methods such as spectrophotometry and high-performance liquid chromatography (HPLC). Six different flavonoids were identified in extracts. The largest amount of phenolic compounds (mainly rutin and quercetin) was found in the intensive growth vegetation stage, and antioxidant activity corresponds with this result. The conducted analysis shows the dependence of phytochemical composition on the vegetation stage when the plant was collected. These results support the use of bur marigold extracts in pharmaceutical or food industry as a potential source of natural inhibitors of PLA₂. Biochemistry analysis using the pull-down method shows that 7 proteins which bind sPLA₂ were found in healthy blood serum and after myocardial infarction. The biggest fraction was albumins. According to the variant of the sample, different proteins are bound to PLA₂. The data of the pull-down analysis correspond with phytochemical analysis, i.e., they support the presence of natural inhibitors of PLA₂ in Bidens tripartita extract.

DOI: http://dx.doi.org/10.5755/j01.erem.72.4.16494