The Effect of Tin Compounds on the Lipid Peroxidation Level of Russian Sturgeon Fresh and Cryopreserved Sperm
Keywords:Russian sturgeon fresh and cryopreserved sperm, organotin compounds, lipid peroxidation, cryomedium
The effect of organotin compounds (OTs) on the accumulation of the lipid peroxidation (LPO) carbonyl by-products, which react with thiobarbituric acid (TBARS) in fresh and cryopreserved sperm of Russian sturgeon, was studied. It was found that incubation (1 hour) of Russian sturgeon sperm with OTs (CH3SnCl3, (CH3)2SnCl2, (CH3)3SnCl, (n-C4H9)2SnCl2, (n-C4H9)3SnCl, (C6H5)2SnCl2, (C6H5)3SnCl) in concentration 0.1 mM led to the promotion of the accumulation of TBARS in native semen. Dimethyl- (DMT) and diphenyltin dichlorides (DPT) exhibited the greatest promoting activity, and the LPO level of both native and cryopreserved sperm of Russian sturgeon, including those in modified Stein's cryomedium, increased in the presence of these compounds. It was found that Russian sturgeon's cryopreserved sperm had lower sensitivity to the promotion of sperm LPO by DMT and DPT compared with the native sperm. The protective effect of Stein’s cryomedium decreased in the presence of the studied OTs. The results suggest that accumulation of OTs by gonad of fish is another stress factor affecting the cell productivity in the cryopreservation process.
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